Product Descripition

This product is a specialized 2X pre-mix designed for qPCR reactions using an improved SYBR Green Ⅰ hybridization fluorescence method. It contains antibody-modified, heat-activated Taq DNA polymerase, dNTPs, a specially optimized buffer, along with unique PCR-enhancing factors and a refined hybrid fluorescent dye. Featuring high specificity, exceptional detection sensitivity, robust fluorescence signals, and rapid reaction kinetics, this product is ideal for quantitative PCR assays targeting amplicons of varying lengths, GC content, and secondary structures. Additionally, it delivers reliable standard curves across a broad quantification range, enabling accurate and precise quantification of target genes with excellent repeatability and high credibility. The product is fully compatible with fast reaction protocols—amplifying products up to 200 bp can even utilize a rapid 10-second extension step, significantly cutting down reaction time while maintaining strong fluorescence intensity. To perform the experiment, simply add your reaction template, primers, and sterile water, making it incredibly convenient to use.

Advantages

Easy to Use: The pre-mixed 2X Premix product is simple to handle, allowing for quick and efficient experimentation.

Ultra-high fluorescence signal and signal-to-noise ratio: Amplification yield is significantly boosted, and when combined with the improved chimeric fluorescent dye, it delivers exceptionally strong fluorescence signals along with an optimal signal-to-noise ratio.

High sensitivity and high specificity: Specially optimized buffer, along with unique PCR enhancers and an improved chimeric fluorescent dye, effectively prevent the formation of primer dimers and non-specific amplification.

It is compatible with rapid PCR reaction conditions, reducing reaction time while maintaining fluorescence intensity.

 Product Components

Experimental Case

Lower Ct values correspond to higher fluorescence intensity.

After 10-fold serial dilutions of both Gene A and Gene B, amplification was performed, and our company's uGreener Fast qPCR 2X Mix was compared with products from Company V and Company T. Our product features a specially optimized PCR reaction system that delivers excellent quantification accuracy and stronger fluorescence signals, ensuring robust amplification even for low-abundance templates.

Superior stability

Place the uGreener Fast qPCR 2X Mix at 37°C for an accelerated experiment, using Gene A and Gene B as templates. After 24 hours and 72 hours, perform the experiments separately and compare them with the control group. 

A Gene Acceleration Experiment

B-Gene Acceleration Experiment

Storage

Store away from light and keep at -20°C for long-term storage. Once thawed, transfer to 4°C and use within 6 months.