Product Descripition

This product is a high-fidelity DNA enzyme premix formulated with a unique composition, containing essential components such as dNTPs and a buffer system. It ensures efficient amplification of long DNA fragments with exceptional fidelity, enabling ultra-fast amplification at a rate of just 5 seconds per kilobase. Its distinctive design and specialized additives enhance the affinity for DNA templates, while its robust 3’→5’ exonuclease activity guarantees unparalleled accuracy during DNA amplification. In addition, this product boasts outstanding features like rapid amplification speed, high sensitivity, and superior fidelity—all combined in a convenient 2X premix format. When using, simply add your primers and template to the reaction mix, eliminating the need for complex preparation steps. This streamlined approach not only simplifies experimental procedures but also minimizes human error, boosting both experimental throughput and the reproducibility of your results.

Product Components

Application

This product is suitable for gene cloning, amplification of complex DNA templates, multiplex PCR, and high-throughput library preparation.

Storage

Store at -20±5°C for 24 months (see label for production date); transport at ≤-20°C.

Exp erimental case

1.Comparison of Enzymes for NGS

（1）The yield and fragment length of 200bp fragmented DNA libraries

The results show:

The yield of the libraries：The yield of T4 (U&G P0016) is comparable to that of product C (the corresponding product from the leading company),the yield of product C (the corresponding product for the leading company) is slightly higher than that of T3 ( U&G P0017 ).

Fragment length：The fragment lengths of T3 ( U&G P0017 ) and T4 ( U&G P0016 ) are comparable to that of product C ( the corresponding product from the leading company ).

（2) The yield and fragment length of 300bp fragmented DNA libraries

The results show:

The yield of the library：The yield of T4 (U&G P0016) is significantly higher than that of the other samples.

The fragment length: there is no significant difference among the 6 samples.

2.Comparison of sequencing data quality of libraries

(1)Analysis of GC distribution pattern

Libraries from 200bp Fragmented DNA	libraries from 300bp Fragmented DNA

(2)Analysis of libraries quality parameters

The results show:

The sequencing data quality of the NGS libraries amplified by U&G P0016 is basically equivalent to that of the control ( the corresponding product from the leading company ).

3.Demonstration of amplification performance

(1)High sensitivity

Using λDNA as the template, with addition amounts ranging from 10 pg to 0.1 fg, the reaction was carried out using 2X GeniuSTAR Max Fast PCR Master Mix (Code: P0016). The results showed that this product had good sensitivity and could stably amplify as low as 1 pg.

M:DL15000 Marker; lane 1 to lane 6： the template amounts are 10 pg, 1 pg, 100 fg, 10 fg, 1 fg, and 0.1 fg respectively;; Lane 7: The negative control is set with dH₂O.

(2)Good stability

The product 2X GeniuSTAR Max Fast PCR Master Mix (Code: P0016) was stored at 4℃ for 14 days, 28 days, and 56 days respectively, and was compared with the product stored under normal conditions. The amplification was performed using a high GC template (GC% = 74%). The results showed that both the treated products and the control could stably amplify the high GC template, and there was no obvious deterioration trend.

M: DL15000 Marker; Lane 1 to Lane 4 represent the product being stored at the recommended temperature, treated at 4°C for 14 days, 28 days, and 56 days respectively.