Product Descripition

The GeniuScript III RT Kit for qPCR (gDNA Removal) is a reverse transcription kit designed specifically for RT-qPCR. It includes reagents that enable the one-step removal of genomic DNA, allowing the reverse transcription reaction to be completed in as little as 20 minutes. The 5X gDNA Removal Mix boasts robust DNA-degrading activity, effectively and rapidly eliminating gDNA from RNA samples within just 2 minutes at 42°C. The 5x GeniuScript III RT Master Mix contains all the essential components required for the RT reaction, including GeniuScript III RTase, RT Buffer, Oligo dT primers, random hexamers, and RNase Inhibitor. This product leverages the highly heat-stable GeniuScript III RTase enzyme along with an optimized buffer system, ensuring efficient reverse transcription across a broad dynamic range of target RNA concentrations. The resulting cDNA products are compatible with both dye-based and probe-based qPCR assays, enabling reliable singleplex and multiplex qPCR reactions. As a result, this kit delivers high-performance gene expression analysis, making it an ideal choice for accurate and sensitive quantitative PCR applications.

Advantages

• Simple to operate—just add template RNA and RNase-free H₂O. 
• It has strong extension capability, can withstand higher reaction temperatures, and is particularly well-suited for RNAs with complex secondary structures.
• Capable of rapidly and efficiently removing gDNA from RNA samples.

Product Components

Experimental Case

Outstanding reverse transcription performance

To evaluate the performance of the GeniuScript III RT Kit for qPCR (gDNA removal), we used HL60 RNA as a template, prepared four 10-fold serial dilutions, and performed reverse transcription (starting at a concentration of 100 ng/μl). Subsequently, equal volumes of cDNA were subjected to qPCR analysis. The results demonstrated that even at low concentrations, the samples still yielded reliable detection, with high fluorescence intensity observed (Figure 1A). Additionally, the resulting standard curve closely matched the ideal profile (Figure 1B).

Figure 1 A

Figure 1 B

Excellent DNA contamination removal properties

DNA removal efficiency was tested using the GeniuScript III RT Kit for qPCR (gDNA Removal), with λ-DNA as the template. After performing four 10-fold serial dilutions of the template, reverse transcription was carried out starting at an initial concentration of 5 pg/μL. Subsequently, equal volumes of the resulting cDNA were used in qPCR experiments. The results demonstrated that, under identical reaction conditions, our product effectively degraded gDNA present in the template—no signal was detected in any of the samples (Figure 2A). In contrast, when the gDNA removal step was omitted as a control, all samples yielded detectable results, with significantly higher fluorescence intensities observed (Figure 2B).

Figure 2 A

Figure 2 B

Comparison of Similar Products

Using λ-DNA as a template, four 10-fold serial dilutions were performed (starting at 500 fg/μL), followed by amplification. The RT reactions were simultaneously conducted using products from our company, T Company, and V Company. Subsequently, Real Time PCR was carried out with our company’s uGreener Flex qPCR 2X Mix (Code. Q0005A), and the results were compared. The findings clearly demonstrate that, under identical reaction conditions, our company’s specially optimized PCR system effectively eliminates gDNA contamination from the template—no detectable signal was observed across all concentration levels. This highlights the superior DNA-degradation capability of our product in minimizing unwanted DNA contamination.

	Fluorescence Curve Graph
Unprocessed Group
U&G
T Company
V Company

Storage

Store away from light at -20℃.