Product Descripition

This product is a high-fidelity DNA polymerase premix formulated with a specialized recipe, containing essential components such as dNTPs and a buffer system. It ensures efficient amplification of long DNA fragments with exceptional fidelity, enabling ultra-fast amplification at a rate of just 5 seconds per kilobase. The premix is highly versatile, performing well on standard templates, challenging high-GC systems (whether involving primers or templates), and even templates containing uracil. Key to its performance is the introduction of an optimized Hot Start high-fidelity DNA polymerase, whose unique design and proprietary additives enhance its affinity for template DNA. Combined with robust 3’→5’ exonuclease activity, this enzyme guarantees unparalleled accuracy during DNA amplification. At the same time, the product delivers outstanding advantages, including rapid amplification speed, superior sensitivity, and excellent fidelity. This product comes as a dye-containing 2X Mix, meaning all you need to do during your experiment is add your template and primers to initiate amplification. After the reaction is complete, there’s no need to add an extra Loading Buffer for visualization—simply proceed directly to sample loading for quick downstream analysis. Plus, thanks to its streamlined workflow, you can skip traditional purification steps altogether, significantly boosting experimental efficiency.

Product Components

Application

This product is suitable for gene cloning and amplification of complex DNA templates (such as high-GC templates or uracil-containing templates).

Storage

Store at -20±5°C for 24 months (see label for production date); transport at ≤-20°C.