High-Fidelity Enzyme Product Selection Guide


High-Fidelity PCR

Product Name

2X GeniuSTAR Max Fast PCR Master Mix

2X GeniuSTAR HiFi Fast PCR Master Mix (Dye plus)

GeniusDirect PCR Kit

Product Catalog Number

P0016A

P0016C

P0017A

P0017C

P0018A

P0018C

Features

Extension Length

Human gDNA

40kb

40kb

10kb

10kb

10kb

10kb

cDNA

14kb

14kb

10kb

10kb

10kb

10kb

λDNA

40kb

40kb

16kb

16kb

20kb

20kb

Extension Time (/kb)

5s-10s

5s-10s

5s

5s

30s

30s

Fidelity (taq)

83X

83X

83X

83X

52X

52X

Product End Structure

Blunt End

Blunt End

Blunt End

Blunt End

Blunt End

Blunt End

Annealing Temperature

60℃

60℃

60℃

60℃

68℃

68℃

GC Compatibility Range

20-80%

20-80%

20-80%

20-80%

30-70%

30-70%

5‘-3’ Exonuclease Activity

None

None

None

None

None

None

Applications

Gene Identification

Molecular Cloning

Recommended for High GC

Supports Amplification of Repetitive Sequences

Other Features

Electrophoresis Indicator

 

 

 

 

Single Enzyme / Premix

Premix

Premix

Premix

Premix

Single Enzyme

Premix